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Operates uploaded in guide manner have their information right handed to bcl2fastq without having additional processing.
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The MiniSeq Fast made a recipe transform that takes advantage of the grafted oligo with the i5 index priming, so it is study inside the forward orientation similar to a MiSeq.
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Seek advice from Index 2 (i5) Orientation To learn more on how to enter i5 bases within the sample sheet in ahead or reverse complement orientation.
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Established C Index Adapters Refer to Index 2 (i5) Orientation To find out more regarding how to enter i5 bases over the sample sheet in ahead or reverse complement orientation.
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When assessing index sets for shade balance, the sequence noticed because of the instrument have to be assessed, regardless of the orientation used for the sample sheet. With the i7 index, generally asses colour balance utilizing the "i7 bases for sample sheet" column orientation.
The sequences displayed are what exactly is entered into the final sample sheet. Kit definitions have the i5 within the ahead orientation. The i5 sequences will probably be appropriately reverse complemented and shown inside the IEM interface dependant on instrument/reagent type.